Examine This Report on Ligaz Football
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), which is identical angular position as being the covalent connections between the ChVLig OB area as well as strands from the latch. As a result, Character has identified very various structural solutions to the trouble of DNA envelopment by ATP-dependent ligases.
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). While in the cost-free ChVLig-AMP, the OB area is reflected far from the NTase domain to totally expose the DNA-binding surface area earlier mentioned the AMP-binding pocket. The peptide section which is destined to become the latch is disordered during the absolutely free ligase and sensitive to proteolysis, but this segment is protected against proteolysis when ChVLig binds to nicked DNA.
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